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Literature summary extracted from

  • Dechene, M.; Wink, G.; Smith, M.; Swartz, P.; Mattos, C.
    Multiple solvent crystal structures of ribonuclease A: an assessment of the method (2009), Proteins, 76, 861-881.
    View publication on PubMed

Crystallization (Commentary)

EC Number Crystallization (Comment) Organism
4.6.1.18 multiple solvent crystal structures, vapor diffusion, hanging drop, PEG 4000, sodium citrate, pH 5.0, temperature 291 K, crystal structure of crosslinked ribonuclease A, resolution 1.65 A, crosslinked crystals are then transferred with a cryo-loop to new drops containing stabilization buffer and an organic solvent and allowed to soak for 1-2 h at room temperature. Soaked crystals are then collected, cryo-protected by dunking in stabilization buffer containing 20% glycerol, and flash frozen in liquid nitrogen, crystal structure of ribonuclease A in 50% dimethylformamide, resolution 1.84 A, crystal structure of ribonuclease A in 50% dioxane, resolution 1.95 A, crystal structure of ribonuclease A in 70% dimethyl dulfoxide, resolution 1.76 A, crystal structure of ribonuclease A in 70% 1,6-hexanediol, resolution 2.00 A, crystal structure of ribonuclease A in 70% isopropanol, resolution 2.02 A, crystal structure of ribonuclease A in 70% t-butanol, resolution 1.68 A, crystal structure of ribonuclease A in 50% trifluoroethanol, resolution 1.93 A, crystal structure of ribonuclease A in 1 M trimethylamine N-oxide, resolution 1.68 A, crystal structure of ribonuclease A in 50% R,S,R-bisfuranol, resolution 1.76 A, comparison of the multiple solvent crystal structures with inhibitor-bound crystal structures of ribonuclease A reveals that the organic solvent molecules identify key interactions made by inhibitor molecules, highlighting ligand binding hot-spots in the active site, investigation of plasticity, hydration and clustering of organic solvent molecules in the active site Bos taurus

Organism

EC Number Organism UniProt Comment Textmining
4.6.1.18 Bos taurus P61823
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-

Purification (Commentary)

EC Number Purification (Comment) Organism
4.6.1.18
-
 Bos taurus

Source Tissue

EC Number Source Tissue Comment Organism Textmining
4.6.1.18 commercial preparation
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 Bos taurus
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Synonyms

EC Number Synonyms Comment Organism
4.6.1.18 bovine pancreatic ribonuclease A
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 Bos taurus
4.6.1.18 RNase A
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 Bos taurus